New Promega HiBiT Protein Tagging System Easily Combines with CRISPR/Cas9 Knock-in to Simplify Studies of Proteins Under Natural Expression Conditions

26-Sep-2017 Intellasia | BusinessWire | 10:12 PM Print This Post

MADISON, Wis.–(BUSINESS WIRE)–#CRISPR–Promega Corporation’s new bioluminescent HiBiT
Protein Tagging System
can be combined with CRISPR/CAS9-mediated
gene editing to tag endogenous proteins and simplify their study under
natural expression conditions, as demonstrated in “CRISPR-Mediated
Tagging of Endogenous Proteins with a Luminescent Peptide,”
a
recently published paper in the journal ACS Chemical Biology.
HiBiT technology, which provides a small, sensitive, easily quantifiable
peptide tag, allows researchers to make better cell models, hastening
efforts in understanding the biology of key human diseases.

“HiBiT really represents a breakthrough in the way we measure protein
trafficking or protein secretion,” says Julian Sebag, PhD, University Of
Iowa Carver College Of Medicine, who is using the HiBiT tag for research
in developing novel treatments for obesity and diabetes. His research
using HiBiT was published last week in BBA
Molecular Cell Research
.

Kentaro Oh-hashi, PhD, Gifu University, Japan, recently demonstrated
CRISPR-mediated knock-in of HiBiT to study ATF4 protein biology
resulting in the publication of a paper last month in Biochemistry
and Biophysics Reports.

HiBiT enables sensitive detection of tagged proteins down to endogenous
expression levels. The small 11 amino acid HiBiT tag is added to a
protein of interest, which can then be detected and quantified with a
simple luminescent signal. The tag’s small size makes it easy to insert
using CRISPR/Cas9 gene editing, and when combined with the detection
reagent, the resulting bioluminescence is sensitive enough to measure
endogenous protein expression in minutes without the need for antibodies.

Promega launched the new HiBiT
Protein Tagging System
in August and it is already gaining global
attention, receiving a 2017 Product Innovation Award at MipTec,
a leading European exhibition highlighting the latest advances in drug
discovery.

“We are excited that the simple approach of using HiBiT with CRISPR/Cas9
allows researchers to more easily get at the true biology being studied
to understand the protein dynamics that are a part of normal physiology
or development of disease,” says Marie Schwinn, Promega Senior Research
Scientist and lead author of the Promega paper in ACS Chemical Biology.

To learn more about the new HiBiT Protein Tagging System visit: promega.com/ProteinTag

About Promega

Promega Corporation is a leader in providing innovative solutions and
technical support to the life sciences industry. The company’s 3,500
products enable scientists worldwide to advance their knowledge in
genomics, proteomics, cellular analysis, drug discovery and human
identification. Founded in 1978, the company is headquartered in
Madison, WI, USA with branches in 16 countries and over 50 global
distributors. For more information about Promega, visit www.promega.com.

Contacts

Promega Corporation
Penny Patterson
Sr. Director,
Communications
(608) 274-4330
penny.patterson@promega.com

 


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